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  • Quantify activation of AP-1, ER alpha, GATA1, GR, HIF-1 alpha, MEF-2, NFkBp50, NFkBp65, p53, PPAR alpha, PPAR gamma, SP-1, STAT1, SMAD-2, SMAD-4, FKHR, EGR-1, HNF-3beta, and E2F-1
  • Detect activation with as little as 0.5 µg of nuclear extract
  • Complete assay in less than 3.5 hours

Affymetrix TF ELISA Kits are ideal for identifying transcription factor binding activity to DNA. The ELISA-based kit combines a fast, user-friendly format with a sensitive and specific assay. TF activation can be detected with as little as 0.5 µg of nuclear extract and the assay completed in less than 3.5 hours. The 96-well plate format, with individual strips of 8 wells, is suitable for manual use or high-throughput screening applications. The technology has many applications including the study of drug potency, inhibitor or activator proteins, and/or protein structure/function in the signaling pathway.

Here's How It Works

In this illustration, activated NFκB p50 molecules from nuclear extracts bind to an NFκB consensus binding site (NFκB Probe) on a biotinylated oligonucleotide. These oligonucleotides are then immobilized on a streptavidin coated 96-well plate. The NFκB p50, bound to the oligonucleotide, is detected by an antibody directed against NFκB p50. An additional horseradish peroxidase (HRP)-conjugated secondary antibody provides a sensitive colorimetric readout quantified by spectrophotometry.