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Cancer Antigen ELISA Kits


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Quickly quantify cancer antigen concentrations in human serum

  • Easy-all-in-one system, no additional equipment needed
  • Efficient-test up to 96 samples at once
  • Rapid-results in less than four hours

Affymetrix now offers four ELISA tests for quantitative determination of tumor marker concentration. Each of the Cancer Antigen ELISAs uses a pair of specific antibodies directed against a unique antigenic determination of each marker-one immobilized on the wells for capturing the cancer markers on the test sample and another linked to horseradish peroxidase (HRP) for detection. The presence of the tumor marker in the serum sample is sandwiched between the captured and the enzyme-linked antibodies and can be detected by using an enzymatic reaction of the HRP conjugated to the secondary antibody. Following color development, the concentration of tumor marker is directly proportional to the color intensity measured at 450 nm.

This technology allows you to quickly and efficiently screen multiple human serum samples for various antigens shown to be associated with different cancers when present at elevated levels. The four assays Affymetrix offers are for Alpha-fetoprotein and Cancer Antigens CA125, CA15-3, and CA19-9.


Elevation of serum AFP to abnormally high values occurs in several malignant diseases—including nonseminomatous testicular cancer and primary hepatocellular carcinoma—and some benign ones, including hepatitis and cirrhosis.


Cancer Antigen 125 (CA125) is a surface antigen associated with epithelial ovarian cancer, and to date CA125 is the most sensitive marker for residual epithelial ovarian cancer. CA125 may also be elevated in patients with lung, cervical, fallopian tube, and uterine cancer and endometriosis.


Cancer antigen 15-3 (CA15-3) is useful for monitoring breast cancer patients post-operatively for recurrence, particularly metastatic diseases. CA15-3 has been shown to be useful in early detection of relapse of ovarian cancer. CA15-3 levels are also increased in colon, lung, and hepatic tumors.


Serum CA19-9 level is frequently elevated in subjects with certain gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. A persistently rising serum CA 19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA 19-9 value may be indicative of a favorable prognosis and good response to treatment.

How the Cancer Antigen ELISA Works


In this illustration, activated the specific cancer antigen antibody is coated on the bottom of the microtiter well. The sample is added to the well and incubation followed by washing, an additional horseradish peroxidase (HRP)-conjugated detection antibody against the specific cancer antigen will bind the complex, providing a sensitive colorimetric readout quantified by spectrophotometry. The concentration of cancer antigen is directly proportional to the color intensity measured at 450 nm.