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Biowave II


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UV/Visible Spectrophotometer

The Biowave II diode array spectrophotometer offers all the benefits described for the Lightwave II with the addition of key life science applications. There are pre-defined methodologies for nucleic acid quantification (DNA, RNA and oligionucleotides), protein assays (BCA, Biuret, Bradford and Lowry) and for cell culture density measurements. The visualision of the nucleic acid scan is particularly useful, especially for RNA samples where impurities may be present in the 230 nm region, yet not have an adverse effect on the A260/A280 ratio. The system is compatible with disposable low volume UV cuvettes The combination of the life science methods with the rapid scanning, kinetics and concentration capabilities of the Biowave II make it a very useful addition to any molecular biology laboratory. In kinetics mode, the basic plot of absorbance against time may be supplemented with the result for äA/min plus the correlation coefficient is also calculated for the duration of the assay. This slope may be multiplied automatically by a factor to convert it directly to rate of reaction. Once again, all results may be printed to an optional integrated high quality printer for a permanent record or via either a USB or Bluetooth connection to a suitable PC for storage or printing. The Biowave II+ is a higher resolution 3nm bandwidth version.


  • Split Beam (RBC) UV/Vis Spectrophotometer 190-1100nm (3nm/5nm bandwidth)
  • Pre-programmed methods for DNA, RNA, Oligos, proteins and cell density
  • Nucleic acid scans for purity checking
  • Low volume capability with 10µL min. quartz cells
  • Novel optics with a Xenon source for long lamp lifetime and low running costs
  • Flexible data export optional accessories include Export to PC software, Wireless Bluetooth®, built in printer or SD card Method/Data storage.


Wavelength selection: monochromator
Type: UV/Vis
Wavelength Range: 190 - 1100nm
Wavelength Accuracy (+/-): 2nm
Wavelength Reproducibility (+/-): 1nm
Light source: Xenon (PTR)
Beam: Split Beam
Optical System: Monochromator Holographic Grating 
Bandwidth: 5nm (3nm Biowave II+) 
Detector: 1024 CCD array
Photometric Range: -0.300 to 2.500A
Photometric Accuracy: +/- 0.003A at 0-0.5A at 546nm
Stability: 0.001A/h at 340nm
Spectral Scanning: yes
Measurement Type: Fixed Wavelength (Abs/%T), Abs ratio, concentration (standard curve), concentration (factor), multiwavelength, kinetics, Life Science Methods, cell density 
Cell Compatibility: Cuvette 10mm, Microcell* , Ultra Microcell*
Other Sample Containers: n/a
Cell Holder: Single Cell
Accessory Options: Printer
Method Storage: Up to 90
Regulatory Compliance: CE
Printer: Thermal built in*, External via Export*
Display: Graphical LCD
Export/ Data Storage: SD card*, Bluetooth*, USB cable* 
Data Interface: USB
Languages: English
Power Requirements: 90-250, 50/60 Hz, 30VA
Weight: 4.5Kg (9.9lb)
Dimensions: 10 x 26 x 39 cm (3.9 x 10.2 x 15.3 inches) 
Certification: CE